Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Potential Role of CHI3L1+ Astrocytes in Progression in MS

doi: 10.1212/NXI.0000000000000972

Figure Lengend Snippet: (A) NfL levels in the CSF increased significantly in patients with MS in the context of disease activity alone. In remission, the levels of NfL in the patients with ADEM differed significantly to those in the patients with MS and NMO. (B) Increased CHI3L1 levels in the CSF of patients with MS, NMO, and ADEM during disease activity, and they were higher in the NMO and ADEM samples than in those from patients with MS. The levels of CSF CHI3L1 in ADEM were significantly higher than in the remission phase. (C) There was significantly more CHI3L1 in progressive MS (PMS) irrespective of disease activity. (D) There was a trend toward more CHI3L1 in patients with PMS than in patients with NMO and ADEM in remission. (E) NfL levels in the CSF were associated with radiologic activity, with a trend toward an association with CHI3L1. (F) The average number of Gd+ lesions in MS and the distribution of these lesions in patients with high levels of NfL and CHI3L1 (oNfL/oCHI3l1) or high NfL and low CHI3L1 (oNfL). Median levels of NfL and CHI3L1 in RMS outside relapse are represented as a red line for reference. A p value of <0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001). ADEM = acute disseminated encephalomyelitis; CHI3L1 = chitinase 3–like 1; Gd+ = gadolinium-enhanced lesion; NfL = neurofilament light protein; NMO = neuromyelitis optica; oCHI3L1 = outlier CHI3L1; oNfL = outlier NfL; RMS = relapsing MS.

Article Snippet: Sections were incubated with a CHI3L1 antibody (AF2599, R & D Systems) diluted in Dako buffer (S3006, Dako, Agilent, Santa Clara, CA), with 10% horse serum to block nonspecific protein binding, whereas the rest of the antibodies were diluted in Dako buffer containing 10% fetal calf serum.

Techniques: Activity Assay

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Potential Role of CHI3L1+ Astrocytes in Progression in MS

doi: 10.1212/NXI.0000000000000972

Figure Lengend Snippet: Light microscope micrographs of AMS and RRMS specimens (A–I). NAWM (A–C). CHI3L1 expression was not found in the NAWM of controls (A), whereas diffuse expression was detected in patients with AMS (B) and RRMS (C). Cortex (D–F). Similarly, no CHI3L1 expression was detected in control specimens (D), whereas it was weakly expressed in the cortex of patients with AMS (E) but not patients with RRMS (F). Active lesion (G–I). A typical early active white matter lesion in a patient with AMS with profuse perivenular infiltrates of CD68 + cells (H) and demyelination, as shown by Klüver-Barrera staining (G). CHI3L1 expression (I) was intense within the lesion, expressed in the center by round cells that correspond to microglia, and at the periphery or active border by larger ramified cells corresponding to astrocytes (arrows in I). CHI3L1, chitinase 3–like 1; AMS = acute MS; NAWM = normal-appearing white matter; RRMS, relapsing-remitting MS.

Article Snippet: Sections were incubated with a CHI3L1 antibody (AF2599, R & D Systems) diluted in Dako buffer (S3006, Dako, Agilent, Santa Clara, CA), with 10% horse serum to block nonspecific protein binding, whereas the rest of the antibodies were diluted in Dako buffer containing 10% fetal calf serum.

Techniques: Light Microscopy, Expressing, Control, Staining

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Potential Role of CHI3L1+ Astrocytes in Progression in MS

doi: 10.1212/NXI.0000000000000972

Figure Lengend Snippet: (A) Quantification of CHI3L1 expression, measured by the OD of staining in different areas of all MS and control specimens. (B) Density of CHI3L1+ cells in each area and type of lesion. (C) Density of CD68 + cells in the distinct types of lesions. (D) There was a positive correlation between CHI3L1 and CD68 expressing cells in all the regions analyzed. (E) There was a positive correlation between perivascular infiltrates and CD68 + cells but not with CHI3L1-expressing cells (not shown). CHI3L1 density: AL cortex vs IL cortex ( p = 0.053), AL WM vs IL WM ( p = 0.083), and SEL edge vs SEL core ( p = 0.053). *CD68 density: AL cortex vs IL cortex ( p = 0.000), AL WM vs IL WM ( p = 0.083), and SEL edge vs SEL core ( p = 0.053). AL = active lesion; AMS = acute MS; CHI3L1 = chitinase 3–like 1; IL = inactive lesion; OD = optical density; PMS = progressive MS. SEL = slow-expanding lesion; WM = white matter.

Article Snippet: Sections were incubated with a CHI3L1 antibody (AF2599, R & D Systems) diluted in Dako buffer (S3006, Dako, Agilent, Santa Clara, CA), with 10% horse serum to block nonspecific protein binding, whereas the rest of the antibodies were diluted in Dako buffer containing 10% fetal calf serum.

Techniques: Expressing, Staining, Control

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Potential Role of CHI3L1+ Astrocytes in Progression in MS

doi: 10.1212/NXI.0000000000000972

Figure Lengend Snippet: Light microscopy micrographs of active and inactive lesions in patients with PMS. Active lesions in the WM (left column), cortex (medium column), and SEL (right column) (A–L). All active lesions displayed extensive demyelination, evident as a loss of reactivity to PLP (A–C), intense inflammatory infiltrates of CD68 + cells (D–F), and an accumulation of APP in transected axons and spheroids as a sign of neurodegeneration (G–I). A detail of the spatial relationship between CHI3L1+ astrocytes and APP+ spheroids is shown as a subset in H; APP: red, GFAP: white, CHI3L1: green, DAPI: blue. CHI3L1 expression was less diffuse and more restricted to the expanding border of the lesions (arrows), and most cells were morphologically identified as astrocytes (see details in J–L). Scale bar: 100 µm (A–L), 20 µm (details J–L); 10 µm (detail in H). APP = amyloid precursor protein; CHI3L1 = chitinase 3–like 1; GFAP = glial fibrillary acidic protein; PLP = myelin proteolipid protein; PMS = progressive MS; SEL = slow-expanding lesion; WM = white matter.

Article Snippet: Sections were incubated with a CHI3L1 antibody (AF2599, R & D Systems) diluted in Dako buffer (S3006, Dako, Agilent, Santa Clara, CA), with 10% horse serum to block nonspecific protein binding, whereas the rest of the antibodies were diluted in Dako buffer containing 10% fetal calf serum.

Techniques: Light Microscopy, Expressing

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Potential Role of CHI3L1+ Astrocytes in Progression in MS

doi: 10.1212/NXI.0000000000000972

Figure Lengend Snippet: Light microscopy micrographs of a case of NMO and of ADEM. NMO (A–H). Active lesions in NMO were devoid of GFAP (A) and AQP4 (B), reflecting astrocyte damage. Loss of PLP reactivity at the active border of the lesion is related to active demyelination (C). The active edge showed CD68 + (D) and CHI3L1 (E) infiltrates. All CHI3L1+ astrocytes had a disrupted morphology as a result of a process referred to as clasmatodendrosis (detail in E). At the inactive center of the lesion, residual demyelination could be observed in the absence of inflammatory cells (F–G), and CHI3L1+ extracellular debris was seen (asterisk in H). Scale bar: 250 μm (A, B); 100 μm (C–J); 10 μm (detail in E and K). ADEM = acute disseminated encephalomyelitis; AQP4 = aquaporin type 4; CHI3L1 = chitinase 3–like 1; GFAP = glial fibrillary acid protein; NMO = neuromyelitis optica; PLP = myelin proteolipid protein.

Article Snippet: Sections were incubated with a CHI3L1 antibody (AF2599, R & D Systems) diluted in Dako buffer (S3006, Dako, Agilent, Santa Clara, CA), with 10% horse serum to block nonspecific protein binding, whereas the rest of the antibodies were diluted in Dako buffer containing 10% fetal calf serum.

Techniques: Light Microscopy